Green Synthesis of Gold Nanoparticles by Leaf Extract
Green Synthesis of Gold Nanoparticles by Leaf Extract
- CHECKLISTFresh leaves of Neem, Bottle Brush or F. Panda, Tetrachloroauric acid (HAuCl4·3H2O), Deionized Water, Beaker, Buret, Magnetic Stirrer with Temperature option, FIlter Paper
- STEP 1.Take fresh leaves of Neem or any other leaves you want. Wash properly and cut them into small pieces.
- STEP 2.Take Leaf pieces (25g) and mix with Deionized Water (100ml).
- STEP 3.Now boil the Leaves at around 80-90ºC for 5-10 minutes.
- STEP 4.When the water turns GREEN, filter it out and use it as reducing and capping agent for Nanoparticles synthesis.
- STEP 5.Take Tetrachloroauric acid (50mg) in deionized water (100ml); and set temperature 80ºC.
- STEP 6.Add leaf extract drop wise until the color of Tetrachloroauric acid solution turns into Red or Violet (Depends on the size of Gold Nanoparticles; further depends on the type of leave you have taken).
- RESULTSThe Red or Violet color indicated the formation of Gold Nanoparticles in water. UV-Vis spectroscopy absorption peak would be obtained around 530nm.
- Factors Affecting SynthesisSpecies of Plant/Tree: Different types of Plants/Trees are having different types of chemical composition and concentration of specific chemical in its leaves. So different species of Plants/Trees can give the different particle size, size distribution. Even morphology can also be changed by using leaf extract of different plants/trees.
Concentration of Tetrachloroauric acid: Tetrachloroauric acid is used as the precursor in this synthesis. Higher the concentration of Tetrachloroauric acid, more the chances of agglomeration; further leads to bulky size particles.
Concentration of Leaves in Water: Concentration of leaves in water is very important for Nanoparticles synthesis. Higher concentration of leaves in water, leads to higher concentration of reducing and capping agent in water.
Temperature: The temperature is another important factor in the synthesis of Nanoparticles, change in temperature leads to change in the particles size; also higher temperature leads to faster chemical Kinetics. - NOTE: All the experiments should be done under the guidance of lab Incharge; and proper lab safety instructions.
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