Synthesis of Silver Nanoparticles by Leaf Extract

CHECK LIST: Fresh leaves of Bottle Brush or F. Panda, Silver Nitrate, Deionized Water, Beaker, Buret, Magnetic Stirrer with Temperature option, FIlter Paper STEP1: Take fresh leaves of Bottle Brush or F. Panda. Wash properly and cut them into small pieces. Raw HTML STEP2: Take Leaf pieces (25g) and mix with Deionized Water (100ml) Raw HTML 500 rpm 60°C rendering="geometricPrecision" viewBox="0 0 640 480" style="white-space: pre;"> STEP3: Now boil the Leaves at around 80-90ºC for 5-10 minutes Raw HTML 500 rpm 60°C after some time 500 rpm 60°C viewBox="0 0 640 480" style="white-space: pre;"> viewBox="0 0 640 480" style="white-space: pre;"> STEP4: When the water turns GREEN, filter it out and use it as reducing and capping agent for Nanoparticles synthesis STEP5: Take Silver Nitrate (10mg) in deionized water (50ml), cover conical flask with Aluminium foil to avoid photo-degradation; and set temperature 60-70ºC Raw HTML 500 rpm 60°C STEP6: Add leaf extract drop wise until the color of Silver Nitrate solution turns light Yellow. Raw HTML 500 rpm 60°C RESULTS: The light yellow color indicated the formation of Silver Nanoparticles in water. UV-Vis spectroscopy absorption peak would be obtained around 420nm.


  • Species of Plant/Tree: Different types of Plants/Trees are having different types of chemical composition and concentration of specific chemical in its leaves. So different species of Plants/Trees can give the different particle size, size distribution. Even morphology can also be changed by using leaf extract of different plants/trees.
  • Concentration of Silver Nitrate: Silver Nitrate is used as the precursor in this synthesis. Higher the concentration of Silver Nitrate, more the chances of agglomeration; further leads to bulky size particles.
  • Concentration of Leaves in Water: Concentration of leaves in water is very important for Nanoparticles synthesis. Higher concentration of leaves in water, leads to higher concentration of reducing and capping agent in water.
  • Temperature: The temperature is another important factor in the synthesis of Nanoparticles, change in temperature leads to change in the particles size; also higher temperature leads to faster chemical Kinetics.

NOTE: All the experiments should be done under the guidance of lab Incharge; and proper lab safety instructions.


  1. I have broad peak between 400-450 for the synthesized silver nanoparticle using plant extract. how can that be rectified?
    I have used 1mM of AgNo3

  2. Dear Aruna,
    Broad peak means the size range of silver nanoparticles is broad. To overcome this problem you can either reduce the amount of Silver nitrate precursor or reducing agent (i.e. leaf extract). You can also try different plants as every plant is having different reduction potential & stabilizing abilities.

  3. What are the chemical equations for this experiment and what is the formula of the green extact

  4. I have synthesized silver nanoparticles from seaweeds. Now i need to test the dry the synthesized silver nanoparticles for performing various biological assay. But I dont know how to dry the synthesized colloidal solution? Kindly give me a suggestion to dyr the particles.

  5. What is the size of the silver nano-particle in nm for this example ?

  6. Prathamesh Gundekar9 February 2019 at 11:37

    But how to separate silver particle in that solution...which precipitated are using..

  7. Hello.
    I have a stem bark extract by Soxhlet extraction. How can I reconstitute it for my AgNP synthesis for best result?
    Thank you in advance.

  8. how can i powder the nanoparticles thus formed